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. 2018 Dec 7;9:1769. doi: 10.3389/fphys.2018.01769

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Copyright © 2018 Karasuyama, Tabakawa, Ohta, Wada and Yoshikawa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Basophils accumulate at the tick-feeding site during the 2^nd but not 1^st tick infestation. Mcpt8^GFP (green basophil) mice were infested with ticks one or twice and subjected to intravital fluorescence imaging analysis of green basophils at tick-feeding sites on day 2 of the 1^st or 2^nd infestation.