Fig. 3.

Size exclusion HPLC A₂₈₀ profiles of 29 nM recombinant (A) apoE2, (B) apoE3, and (C) apoE4 incubated at 35°C with protease inhibitors and 2.59 μM micellar cholesterol. ApoE3 tetramers slowly disaggregated to monomers, whereas apoE4 remained intact, indicating that structural differences among apoE isoforms affect its oligomerization properties. This result shows that apoE3 dissociates faster, indicating differences in binding properties. (Typical results of three to four experiments with each isoform shown.) Abbreviation: apoE, apolipoprotein E.