Table 2.
Effect of cytokines and enzyme inhibitors on apoE particle size in human astrocytes and neuronal cells
| Cells/treatment | Retention time (min) | Diameter (nm) | Molecular weight (kDa) | P value |
|---|---|---|---|---|
| Astrocytes | 6.90 ± 0.09 | 17.7 ± 0.3 | 2160 ± 120 | — |
| Neuronal cells | 7.78 ± 0.02 | 14.6 ± 0.1 | 1200 ± 10 | .0015 |
| Astrocytes + PSC833 | 6.97 ± 0.06 | 17.4 ± 0.2 | 2050 ± 90 | ns |
| Astrocytes + cytokines | 6.10 ± 0.04 | 17.3 ± 0.2 | 2020 ± 60 | ns |
| Astrocytes + oxidized lipids | 6.56 ± 0.05 | 19.1 ± 0.2 | 2680 ± 80 | .027 |
| Astrocytes + 4-hydroxy-nonenal | 7.15 ± 0.07 | 16.7 ± 0.3 | 1830 ± 90 | ns |
NOTE. Retention time was measured by differentiation of the smoothed chromatograms. P values are calculated relative to untreated astrocytes. Oxidized lipids were added at 18 μg/mL and 4-hydroxynonenal was added at 16 μM.