Figure 2.

Pten knockout affects collagen alignment surrounding tumors. (A) H&E staining, second harmonic generation, and picrosirius red staining of murine mammary gland tissue sections containing tumors. SHG images are shown above their corresponding picrosirius red image. Solid lines represent the tumor-stroma boundary. (B) Histogram of collagen fiber angle relative to the tumor edge, quantified using CurveAlign software. Bin size=10°. P value represents the probability of the WT and Pten^−/− histograms originating from the same distribution. (C) Graph of the fraction of fibers oriented at <10° or >80° relative to the tumor edge, shown as mean±SEM. WT;MMTV-Neu: n=12. Pten^−/−;MMTV-Neu: n=15, where n represents the number of tumor regions imaged from 3 separate mice. *P<.05.