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Pten knockout in fibroblasts alters matrix structure. (A) Fibronectin immunostaining of fixed or decellularized FDMs, color-coded by fiber orientation. (B) Histogram of fibronectin fiber orientation from fixed matrices; bin size=1°. (C) Graph of the fraction of fibers in the matrix falling within 10° or 20° of the mode orientation. (D) Height of unextracted matrices. (E) Protein content of solubilized matrices normalized to DNA content. n=3±SEM. *P<.05, **P<.01, ***P<.001.
