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Pten deletion increases fibroblast activation. (A) Immunofluorescence images of WT or Pten−/− MMF cultured for 5 days with ascorbic acid, then fixed and stained for αSMA and nuclei. (B) Western blotting and quantification of PTEN (54 kDa), αSMA (42 kDa), and GAPDH loading control (37 kDa), n=5±SEM. (C) MMP activity of MMF encapsulated in PEG hydrogels, n=3±SEM. (D) Normalized mRNA expression of matrix components as determined by RT-PCR, n=3±SEM. *P<.05, ****P<.0001.
