Figure 5.

Increased contractility in Pten^−/− fibroblasts regulates matrix alignment. (A) Color maps of the traction stress field in WT and Pten^−/− fibroblasts. (B) Maximum traction stress was quantified and plotted as median±95% confidence interval, n=17-22. (C) The net contractile moment of each cell was quantified and plotted as median±95% confidence interval, n=17-22. (D) Fibronectin staining of Pten^−/− matrices treated with contractility inhibitors, pseudocolored by fiber orientation. (E) Alignment of Pten^−/− matrices treated with Blebbistatin (myosin inhibitor) or Y-27632 (ROCK inhibitor) as compared to a DMSO vehicle control. n=3±SEM. *P<.05, **P<.01, ***P<.001, ****P<.0001 relative to control. ^^P<.01 relative to 1-μM concentration of the same inhibitor.