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. 2018 Dec 12;21(1):132–145. doi: 10.1016/j.neo.2018.10.010

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Pten FDMs promote DB7 cancer cell elongation and migration. (A) Staining of DB7 cells plated on glass or FDMs. (B) Quantification of DB7 cell and nuclear area from phalloidin and Hoechst staining using CellProfiler. (C) Quantification of DB7 cell and nuclear aspect ratio using CellProfiler. n=3±SEM. (D) Representative images of DB7 spheroids at time 0 or after 12 hours of imaging. (E) DB7 spheroid spreading as determined by the final area of the spheroid divided by its initial area. (F) Aspect ratio of the spheroids at 12 hours. n=13-16 spheroids across 4 experiments. *P<.05, ***P<.001, *** P<.001, ****P<.0001.