Fig. 6.
Effect of the selective A2B adenosine receptor (A2B AR) antagonist MRS-1754 (M1754) on adenosine (ADO)-induced inhibition of [14C]oxalate uptake by Caco2-BBE (C2) cells. C2 cells were preincubated with vehicle (Control) or 100 µM ADO for 2 min in the culture medium, and then [14C]oxalate uptake was measured as described in materials and methods. [14C]oxalate uptake was also measured in the presence of M1754 (100 µM) for 5 min followed by 100 µM ADO with continued presence of M1754 (ADO + M1754) for 2 min or 100 µM M1754 alone for 7 min. Values are means ± SE of 4 independent experiments each of which was done in triplicate and was normalized to the respective Control value. M1754 significantly reduced the inhibition induced by ADO (*P < 0.001 for ADO and M1754 compared with Control; ^P < 0.05 for ADO compared with ADO + M1754, by ANOVA).