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. 2018 Jul 18;315(5):C687–C698. doi: 10.1152/ajpcell.00024.2017

Fig. 13.

Fig. 13.

Effect of adenosine (ADO) on surface alkaline phosphatase (AKP) protein expression in Caco2-BBE (C2) cells. A: a representative Western blot analysis of surface AKP protein expression. C2 cells were preincubated with vehicle (Control) or 100 µM ADO for 2 min in the culture medium, and then AKP surface protein expression was evaluated after streptavidin precipitation of surface biotinylated proteins from 300 µg of initial cell lysate. The lower half of the same blot was probed with an anti-GAPDH antibody. B: densitometry of immunoblot results. Western blot band density was quantified using ImageJ software. Values are means ± SE for 3 independent experiments and are presented as a percentage of the respective Control value. ADO has no significant effect on AKP surface protein expression.