Figure 4.

Attenuated calcium flux in NHBEs with reduced CARMA3 expression. NHBEs were grown in T-75 flasks and infected with either a lentivirus containing a nontargeting shRNA (scRNA) or a CARMA3-targeting shRNA. NHBEs were trypsinized and incubated with 3 μM Fluo-4. Baseline calcium levels were recorded for 1 minute and were followed by the addition of either (A and B) 2.5 μM A23187 (positive control/Ca^2 + ionophore; Sigma-Aldrich) or (C and D) 100 μg A. alternata. Representative plots are shown from three independent experiments.