Figure 6.

CARMA3 and the ITPRs colocalize in AECs. (A) Immunofluorescence of NHBEs stained with antibodies (Abs) against CARMA3 and ITPR1 (top row), ITPR2 (middle row), and ITPR3 (bottom row). All images were taken using the same exposure at ×60 magnification. (B) In situ proximal ligation assay on NHBEs stained with Abs against CARMA3 and ITPR1 (left), ITPR2 (middle), and ITPR3 (right). The negative control includes a nonspecific Ab instead of the ITPR Abs and the positive control is done with two Abs against CARMA3. All images were taken using the same exposure at ×40 magnification.