Figure 4.

T cells infiltrate lungs with Tsc2-null lesions and highly express PD-1. (A) Lungs with Tsc2-null lesions taken from C57BL/6 mice killed ∼3 weeks after injection have increased CD4⁺ and CD8⁺ T cells, CD4⁺ and CD8⁺ effector/effector memory T (T_EM) cells, and regulatory T cells (T_REGS), presented as a percentage of total CD4⁺ and CD8⁺ T cells. (B) Representative flow-cytometry diagrams illustrate the increase in CD4⁺ and CD8⁺ T_EM cells in lungs with Tsc2-null lesions compared with controls. (C) Quantification of overall PD-1⁺ CD4⁺ and CD8⁺ T cells, and representative plots showing PD-1 expression on T_EM cells in control lungs (red) compared with lungs with Tsc2-null lesions (blue). Statistical analysis was performed using Student’s t test with significance at *P < 0.05 (***P < 0.001, n > 5 mice, n = 2 experiments).