FIG 5.

Metabolite labeling patterns during growth on [U-¹³C₆]glucose (¹³C₆-Glucose) alone or with unlabeled glucose (¹²C₆-Glucose), unlabeled galactose (¹²C₆-Galactose), unlabeled xylose (¹²C₆-Xylose), or unlabeled arabinose (¹²C₆-Arabinose). (A) Carbon mapping (left) and the labeling data (right) for intracellular metabolites in the upper Embden-Meyerhof-Parnas pathway following feeding on [¹³C₆]glucose alone or with [¹²C₆]glucose or [²C₆]galactose. (B) Carbon mapping (left) and the labeling data (right) for intracellular metabolites in the pentose-phosphate pathway following feeding on [¹³C₆]glucose alone or with [¹²C₆]glucose, [¹²C₆]xylose, or [¹²C₆]arabinose. In the carbon mapping in panels A and B, the open circles and the filled circles represent nonlabeled and ¹³C-labeled carbons, respectively. Data were obtained at two time points during exponential growth: at OD₆₀₀ 0.5 to 0.6 (T1) and at OD₆₀₀ 0.9 to 1.0 (T2). Metabolite labeling data (average ± standard deviation) were from independent biological replicates (n = 3). Very small error bars are not noticeable. The metabolite abbreviations are as listed in legends for Fig. 1 and 3.