FIG 8.

Metabolic routing of mannose through central metabolism in the presence of glucose. (A) Kinetic profiling of metabolite labeling patterns in cells grown simultaneously on [U-¹³C₆]glucose and unlabeled mannose; the lines through the data are to guide the eye. (B) Dynamic flux ratio analysis for the metabolic entry point of glucose versus mannose assimilation through G6P and FBP, respectively: flux from mannose (light orange), flux from ED pathway (gray), flux from F6P (green), flux from glucose (blue). Data in panel B were obtained at six time points during exponential growth: at OD₆₀₀ of 0.21 to 0.27 (T1), at OD₆₀₀ of 0.40 to.44 (T2), at OD₆₀₀ of 0.54 to 0.67 (T3), at OD₆₀₀ of 0.79 to.87 (T4), at OD₆₀₀ of 0.90 to 0.98 (T5), and at OD₆₀₀ of 1.2 to 1.4 (T6). In panels A and B, The data (average ± standard deviation) are from biological replicates (n = 3). The metabolite abbreviations are as listed in the legend for Fig. 1.