FIG 1.

Pseudoalteromonas sp. PS47 contains a pectin utilization locus and produces pectinases. (A) Schematic depiction of the pectin utilization loci found in the genomes of Gramella flava JLT2011, Pseudoalteromonas sp. PS47, and Pseudoalteromonas haloplanktis ANT/505. Open reading frames (ORFs) are shown to scale (within a locus) and colored according to putative function. CAZyme family classifications, according to www.cazy.org and/or the dbCAN2 server, are given underneath or above; some of these classifications have been updated (indicated by #) and therefore differ from those originally reported by Tang et al. (29) and Hehemann et al. (31). Light gray bars between CAZyme-encoding ORFs indicate amino acid sequence identities of >40% (see Table 1 for exact sequence identities). Abbreviations: CE, carbohydrate esterase; GH, glycoside hydrolase; PL, polysaccharide lyase; DKI, 5-keto-4-deoxyuronate; DKII, 2,5-diketo-3-deoxygluconate; KDG, 2-keto-3-deoxygluconate. (B) Growth of PS47 on citrus pectin as the sole carbon source in minimal marine medium, with a no-sugar control shown for comparison. Absence of growth on galacturonate (GalUA) is also shown. Data shown are the mean of two biological replicates; error bars represent the SEM. (C) FACE gel showing the presence of pectinases in both the culture supernatant and cell lysate of PS47 grown in Zobell marine medium containing 0.2% pectin. + and −, presence and absence, respectively, of substrate or cell fraction. GalUA and digalacturonate (GalUA₂) were included on the gel as standards.