FIG 2.

PsPL1 displays endolytic lyase activity against a range of pectin-related substrates. (A) FACE gel of pectin and related polysaccharides following overnight incubation with 1 μM PsPL1 in the presence of Ca²⁺. + and −, presence and absence, respectively, of PsPL1; E, enzyme-only sample. Abbreviations: PGA, polygalacturonate; AGU, apiogalacturonan; RGI, rhamnogalacturonan I; ΔGalUA₂, unsaturated digalacturonate (included as a standard). (B) Activity of PsPL1 against oligosaccharides of galacturonate with various degrees of polymerization. + and −, presence and absence, respectively, of PsPL1; E, enzyme-only sample. ΔGalUA₂ and galacturonate (GalUA) are included as standards.