FIG 6.

In vitro reconstitution of the pectin degradation pathway from Pseudoalteromonas sp. PS47. Linked assays in which the rates of DKI (A) and GalUA (B) production from pectin by PS47 enzymes were quantified by following their downstream processing into KDG (by YeKduI and YeKduD, with concomitant oxidation of NADH) and galactarate (by uronate dehydrogenase, with concomitant reduction of NAD⁺), respectively, were performed. Data shown indicate the effect of omitting one or more enzyme. Data are the means of four replicates and have been corrected for background changes in absorbance in the presence of pectin only. Error bars represent the SEM. Asterisks indicate statistically significant differences between enzyme combinations and the “all enzymes” sample as determined by a one-way ANOVA with Dunnett’s posttest (***, P ≤ 0.001; ****, P ≤ 0.0001; absence of asterisks, P > 0.05).