TABLE 2.
Primers used in this study
| Primer name | Primer sequence (5′→3′)a |
|---|---|
| PsPL1 fwd | GCCGCGCGGCAGCCAACTCGACTCAAATTTAGCCTTTAAAAATGC |
| PsPL1 rev | GCTCGAATTCGGATCGATTACTCCGTAATCGAATTTATATAAGC |
| PsCE8 fwd | GCCGCGCGGCAGCCATTGCAACATCCACTCAAAAACACC |
| PsCE8 rev | GCTCGAATTCGGATCTTATAAGCTAAAGCTATCAGTAGC |
| PsCE10 fwd | GCCGCGCGGCAGCCAGAAAGAGTACACGTTGC |
| PsCE10 rev | GCTCGAATTCGGATCTTATAGCTGTTTTTTTAAAAATAACG |
| PsCE12 fwd | GCCGCGCGGCAGCCAGAAACAAGGTACAACACA |
| PsCE12 rev | GCTCGAATTCGGATCTCAACCTTTTAGTTTTAAATGC |
| PsGH105A fwd | GCCGCGCGGCAGCCAAAAAGAAAGCACTGCAGCAT |
| PsGH105A rev | GCTCGAATTCGGATCTTACTTTACTGAGTTATCTATATC |
| PsGH105B fwd | GCCGCGCGGCAGCCACGAGCCATTACCAAATAAAAATTTATG |
| PsGH105B rev | GCTCGAATTCGGATCTTAACGCATCAAAGCAAGGCTAGC |
| PsGH28 fwd | GCCGCGCGGCAGCCAATGTAAATCAGCCCCCCTTG |
| PsGH28 rev | GCTCGAATTCGGATCTTACTGGGTTATTTTAGCTAA |
| PsGH43 fwd | GCCGCGCGGCAGCCAAATGCGACCAATAAATATAATAATACTG |
| PsGH43 rev | GCTCGAATTCGGATCTTAATTTTGCTCTGTTGGTGTGAAACG |
| PsGH105A CAT fwd | GCCGCGCGGCAGCCAAGATGCAAAAAGTGCTTTAAAGTGG |
| PsGH105A DUF rev | CTCGAATTCGGATCTTACAGTGGTGCTTTGAATTGAG |
a
Vector sequences used for In-Fusion cloning are underlined.