Fig. 1.

Knockout of Pdha1 in PDHmKO mice. Transcript levels in gastrocnemius (GA) muscle from PDHmKO and WT mice, normalized to 36b4 (n = 5/5) (A). PDH activity in GA muscle of PDHmKO and WT mice (B). Representative blots of PDHα protein abundance in GA and tibialis anterior (TA) muscle (C), heart and liver (D) of PDHmKO and WT mice. Bar graphs represent quantification of PDHα protein abundance (n = 6/7) in PDHmKO and WT mice, normalized to eEF2. Data reported as means ± SE. Student’s t-test, *P < 0.05 compared with WT. eEF2, eukaryotic translation elongation factor 2; PDHmKO, tamoxifen-inducible Pdha1 knockout mice; WT, wild-type.