Fig. 5.

The effects of impaired adipose tissue lipolysis on fasting-induced increases in hepatic DC/MC content. Male mice containing an adipocyte-specific Atgl deletion (AAKO) were fasted for 16 h (fast) or allowed ad libitum access to food (fed) before analyses. A: plasma nonesterified fatty acids (FFA, n = 4); B: liver triglyceride levels (TG, n = 4); C: liver, adipose tissue, and splenic dendritic cells (cDC, pDC), and monocyte/macrophage (MC) populations as assessed by flow cytometry (n = 4); D: similar populations as in C were assessed in the liver of lean and obese AAKO mice (n = 7); E: liver glutathione (GSH; n = 4). All plots represent mean ± SE and significant differences are indicated (*P < 0.05 AAKO fed vs. AAKO fast). cDC, conventional DC; DC, dendritic cell; FFA, free fatty acid; MCP, monocyte chemoattractant protein; pDC, plasmacytoid DC; NEFA, nonesterified fatty acid.