Fig. 3.

Adipocyte differentiation-related protein (ADRP) localizes to the perimeter of retinoid-containing lipid droplets in hepatic stellate cells (HSCs). Rat HSCs were isolated, grown on glass coverslips, fixed at days 1, 7, and 21, and labeled with ADRP primary antibody followed by fluorescein isothiocyanate (FITC)-labeled secondary antibody. Cells were visualized at 360 nm, which detects retinoids (blue), followed by visualization in the FITC channel (green). In the right column, merge of both channels is shown. Scale bars = 10 μm. Images are representative of >10 others.