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. 2018 Dec 7;8:592. doi: 10.3389/fonc.2018.00592

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Copyright © 2018 Kalamegam, Sait, Ahmed, Kadam, Pushparaj, Anfinan, Rasool, Jamal, Abu-Elmagd and Al-Qahtani.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative phase contrast images of the human umbilical cord Wharton's Jelly Stem Cells (hWJSCs) in early and late cultures (A,B). The monolayer of hWJSCs shows the characteristic appearance as short fibroblasts in early passages, which become long fibroblasts in later passages. (C) Representative histogram of the MSC related CD surface markers on hWJSCs using Flrorescent activated cell sorting (FACS). Top panel (A) shows the respective isotype controls. The middle panel (B) shows the positive MSC related surface CD markers namely CD73, CD90, CD105, CD29, and CD44. The bottom panel (C) shows the MSC negative surface CD markers namely CD34 and CD45. (D,E) Representative phase contrast images of the human ovarian cancer cell lines OVCAR3 (D) and SKOV3 (E) in culture showing the characteristic epitheloid cells.