Figure 1. Cnot6l expression pattern and phenotype of Cnot6l knockout mice.

• A, B
  Quantitative RT–PCR results showing relative expression levels of mouse Cnot6 and Cnot6l in oocytes (GV and MII), somatic tissues, ES cells (A), and preimplantation embryos (B). n = 3 biological replicates.
• C
  Cumulative numbers of pups per female showing fertility of WT and Cnot6l ^−/− female mice. n = 5 females for each genotype.
• D
  Representative image results of oocytes collected from oviducts of WT and Cnot6l ^−/− mice at 16 h after hCG injection. Scale bar, 100 μm. Arrows indicate polar body‐1 (PB1).
• E
  Confocal microscopy results of oocytes collected from oviducts of WT and Cnot6l ^−/− mice at 16 h after hCG injection. Scale bar, 20 μm. Arrows indicate spindle poles.
• F
  Rates of PB1 emission and normal spindle formation in oocytes ovulated by WT and Cnot6l ^−/− female mice. The numbers of analyzed oocytes are indicated (n).
• G
  Quantification of preimplantation embryos derived from WT and Cnot6l ^−/− females that develop to the indicated stages after hCG administration and mated with adult WT males. The numbers of analyzed embryos are indicated (n).
• H
  Representative images of embryos collected from the oviducts or uteri of WT and Cnot6l ^−/− female at indicated time points after hCG administration. Scale bar, 100 μm.

Data information: Error bars, SEM. **P < 0.01; ***P < 0.001 by two‐tailed Student's t‐test. n.s.: non‐significant.