Figure 5. Transcripts related to mRNA translation were degraded during oocyte maturation but were stabilized by Cnot6l knockout.

1. Level changes of transcripts encoding ribosomal proteins in Cnot6l ^−/− oocytes at MII stage. The fold changes (Cnot6l ^−/−/WT) are listed to the right of the boxes.
2. Quantitative RT–PCR results showing the relative levels of indicated transcripts in WT, Cnot6l ^−/−, and Btg4 ^−/− oocytes at GV and MII stages. n = 3 biological replicates. Error bars, SEM. Statistical analysis was performed using Student's t‐test. *P < 0.05, **P < 0.01, ***P < 0.001. n.s.: non‐significant.
3. Changes of qRT–PCR results obtained from oligo‐dT‐ versus random primer‐mediated RT reactions reflecting the poly(A) tail length changes of given maternal transcripts during GV‐to‐MII transitions in WT, Cnot6l ^−/−, and Btg4 ^−/− oocytes. n = 3 biological replicates. Error bars, SEM.
4. Poly(A) tail assay results showing changes in poly(A)‐tail length of indicated transcripts in WT and Cnot6l ^−/− oocytes at GV, MI, and MII stages. Experiments were performed three times with reproducible results; a representative result is shown.