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. 2018 Nov 26;37(24):e99333. doi: 10.15252/embj.201899333

Figure EV5. Role of Zfp36l2 and ERK1/2 in regulating meiotic maturation of mouse oocyte.

Figure EV5

  1. qRT–PCR results showing the efficiency of Zfp36l2 RNA interference in oocytes at the GV stage. n = 3 technical replicates. Error bars, SEM. ***P < 0.001 by two‐tailed Student's t‐test.
  2. GVBD and PB1 emission rates of oocytes microinjected control and Zfp36l2‐targeted siRNAs. Error bars, SEM. *P < 0.05; ***P < 0.001 by two‐tailed Student's t‐test. The numbers of analyzed oocytes are indicated (n).
  3. Confocal microscopy results showing spindle assembly at MI and MII stages in oocytes microinjected with control siRNAs or siZfp36l2. Scale bar, 20 μm.
  4. PB2 emission and spindle assembly in oocytes cultured with or without U0126 treatment. Fully grown GV oocytes were microinjected with mRNAs encoding CNOT6L, CNOT7, and/or BTG4 and are released from milrinone at 12 h after microinjection. Then, the oocytes were further culture for 24 h with or without adding U0126 (20 μM) to the medium. Scale bar, 100 μm in brightfield images; Scale bar, 20 μm in immunofluorescence staining images.