Figure 7. Role of (A+U)‐rich element (ARE)‐binding protein ZFP36L2 in CNOT6L‐mediated maternal mRNA decay.

1. Presence of putative AREs (AUUUA) in transcripts of WT oocyte. Transcripts containing no ARE or transcripts containing 1 or more AREs were subdivided into frequency groups according to the log₂ fold change (MII/GV) in WT oocytes.
2. Presence of putative AREs in oocyte transcripts stabilized by Cnot6l ^−/− knockout. Transcripts containing no ARE or transcripts containing 1 or more AREs were subdivided into frequency groups according to the log₂ fold change (Cnot6l ^−/−/WT) at MII stage.
3. Co‐IP results showing interaction of ZFP36L2 with CNOT6L and CNOT7. HeLa cells were co‐transfected with plasmids expressing HA‐ZFP36L2 and FLAG‐CNOT6L/7 for 48 h before immunoprecipitation. Experiments were performed three times with reproducible results; a representative result is shown.
4. RNA immunoprecipitation results showing interaction of CNOT6L with indicated transcripts, with or without the presence of ZFP36L2. HeLa cells were co‐transfected with plasmids expressing FLAG‐ZFP36L2 and HA‐CNOT6L for 48 h before immunoprecipitation using an anti‐HA antibody. mRNAs recovered from the immunoprecipitates were subjected to qRT–PCR. Error bars, standard deviations (n = 3 biological repeats). ***P < 0.001 by two‐tailed Student's t‐test.

Source data are available online for this figure.