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. 2018 Nov 5;37(24):e99649. doi: 10.15252/embj.201899649

Figure EV1. Demonstrating functional expression of Chronos in SGNs following transuterine AAV2/6 injection reported by recordings of oABRs.

Figure EV1

  • In 3 out of dozens of animals, we could record oABRs using fiber‐coupled laser stimulation 4–12 weeks after embryonic transuterine injection of AAV2/6 carrying Chronos using 4 ms pulse train at 10 Hz with 30 mW radiant flux. Responses were verified as oABRs by the increasing amplitude with stronger light pulses (inset: shows oABRs of an exemplary oABR‐positive mouse for varying radiant flux, colors code the radiant flux in mW). A small onset and offset artifact (arrowheads) is visible for the average (black) of the negative animals (gray) which we occasionally observed in the early phase of the project.