Figure EV1. Dot blot analysis of phospho‐specific antibodies.

1. Dot blot analysis of the MAP1S pSer⁹⁰⁰ antibodies. Increasing amounts of MAP1S pSer⁹⁰⁰ phosphopeptide immunogen and non‐phosphopeptide equivalent were spotted onto nitrocellulose, and membrane was subjected to dot blotting with two different concentrations of affinity‐purified MAP1S pS⁹⁰⁰ antibodies (0.2 μg/ml for 18 h or 1 μg/ml for 1 h). Two different antibody bleeds were tested.
2. Dot blot analysis of CEP131 pSer³⁵ antibodies. Increasing amounts of CEP131 pSer³⁵ phosphopeptide immunogen and non‐phosphopeptide equivalent was spotted onto nitrocellulose. After cross‐linking with glutaraldehyde (1% (v/v) in H₂O for 10 min), the membrane was subjected to dot blotting with two different concentrations of affinity‐purified CEP131 pSer³⁵ antibodies (0.2 μg/ml for 18 h or 1 μg/ml for 1 h). Two different antibody bleeds were tested.

Source data are available online for this figure.