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Anti‐FLAG precipitates from HEK293 cells transiently expressing FLAG‐tagged CDKL5 (wild type “WT” or a K42R kinase‐dead “KD” mutant) were incubated with the synthetic peptides indicated in the presence of [γ‐32P]‐labelled ATP‐Mg2+, and peptide phosphorylation was measured by Cerenkov counting. Data are represented as mean ± SEM from three independent experiments.Source data are available online for this figure.
