Contractility of bladder strips at 6 wk of age. Peak contractile force in response to EFS (left) and CCh (right) (A), KCl (left) and ATP and α,β-meATP (right) (B); N = 10–12 per group. Open and closed bars represent control and NMS groups, respectively. Representative raw traces of contractions in response to EFS and CCh from each group (C). The force was normalized with tissue weight. Means ± SE, **P < 0.005. α,β-meATP, α,β-methylene ATP; CCh, carbachol; EFS, electric field stimulation; NMS, neonatal maternal separation.