Fig. 6.

Expression analyses of neurotransmitter receptors in bladders. A: a significant decrease in mRNA expression levels of muscarinic receptors (Chrm2 and Chrm3) and a significant increase of P2X₁ were detected in bladders from NMS mice compared with control mice. Normalized with Actb (n = 4 per group). B: a decrease in Chrm2 and an increase in P2X₁ protein expression were detected in bladders from NMS mice compared with the control group. Representative Western blotting results (left) and the summary of changes in Chrm2 and P2X₁ at protein level normalized with Gapdh (right) (n = 4 per group). Means ± SE fold difference (control group taken as 1). White and black bars represent control and NMS groups, respectively. *P < 0.05. NMS, neonatal maternal separation.