Table 2.
Renal hemodynamic parameters of rats 24 h or 5 days after ischemia or sham ischemia
| Sham (24 h) |
Ischemia (24 h) |
Sham (5 days) |
Ischemia (5 days) |
2-Way ANOVA |
Dichotomous Comparison |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Parameter | Value | n | Value | n | Value | n | Value | n | PTr | PT | PTr*T | S1 vs. I1 | S5 vs. I5 |
| Renal blood flow, ml/min | 3.5 ± 2.9 | 10 | 3.0 ± 0.4 | 9 | 3.6 ± 0.7 | 7 | 2.4 ± 0.3 | 8 | 0.13 | 0.83 | 0.68 | 0.55 | 0.29 |
| Renal blood flow, μl·min−1·g body wt−1 | 9.09 ± 0.98 | 10 | 7.93 ± 0.79 | 9 | 7.71 ± 1.59 | 7 | 6.65 ± 0.78 | 8 | 0.30 | 0.21 | 0.96 | 0.60 | 0.81 |
| Renal plasma flow, ml/min | 1.7 (1.5, 2.1) | 10 | 1.7 (1.4, 2.0) | 9 | 1.6 (1.5, 3.0) | 7 | 1.6 (0.9, 1.9) | 8 | 0.42 | 0.65 | 0.87 | 0.36 | 0.51 |
| Renal plasma flow, μl·min−1·g body wt−1 | 5.1 ± 0.6 | 10 | 4.9 ± 0.5 | 9 | 4.4 ± 0.9 | 7 | 3.8 ± 0.4 | 8 | 0.77 | 0.31 | 0.94 | 0.96 | 0.80 |
| Glomerular filtration rate, ml/min | 0.8 (0.7, 1.2) | 10 | 0.001 (0, 0.008) | 9 | 1.0 (0.5, 1.8) | 7 | 0.07 (0.01, 0.4) | 9 | <0.001 | 0.17 | 0.11 | <0.001 | 0.14 |
| Glomerular filtration rate, nl·min−1·g body wt−1 | 2,400 (1,520, 2,960) | 10 | 3.3 (0, 2.2) | 9 | 2,110 (1,040, 3,470) | 7 | 190 (27, 1,080) | 8 | <0.001 | 0.18 | 0.03 | <0.001 | 0.20 |
| Urine flow, μl/min | 7.0 ± 1.0 | 10 | 1.0 ± 0.5 | 9 | 10.0 ± 4.0 | 7 | 6.0 ± 2.0 | 8 | 0.01 | 0.09 | 0.99 | 0.001 | 0.36 |
| Urine flow, nl·min−1·g body wt−1 | 16.0 (10.0, 28.0) | 10 | 2.2 (0, 5.5) | 9 | 17.0 (13.0, 25.0) | 7 | 15 (1.1, 39.0) | 8 | 0.04 | 0.05 | 0.06 | 0.002 | 0.66 |
| Sodium excretion, µmol/min | 0.4 (0.2, 1.0) | 9 | 0 (0, 0.18) | 9 | 0.20 (0.2, 0.6) | 7 | 0.20 (0.1, 0.3) | 8 | 0.004 | 0.46 | 0.09 | 0.003 | 0.38 |
| Sodium excretion, nmol·min−1·g body wt−1 | 1.0 (0.5, 2.1) | 9 | 0 (0, 0.47) | 9 | 0.5 (0.36, 1.3) | 7 | 0.6 (0.35, 0.71) | 8 | 0.02 | 0.53 | 0.05 | 0.01 | 0.90 |
| Sodium reabsorption, µmol/min | 111.9 (93.6, 170.2) | 9 | 0.17 (0, 1.0) | 9 | 138.3 (63.3, 250.4) | 7 | 8.75 (1.5, 52.0) | 8 | <0.001 | 0.2 | 0.15 | <0.001 | 0.14 |
| Sodium reabsorption, nmol·min−1·g body wt−1 | 307.5 (204.7, 427.6) | 9 | 0.46 (0, 2.7) | 9 | 310 (143.6, 479.3) | 7 | 25.7 (3.6, 153.1) | 8 | <0.001 | 0.18 | 0.03 | <0.001 | 0.20 |
| Filtration fraction, % | 46.9 (34.1, 69.8) | 10 | 0.1 (0, 0.5) | 9 | 48.5 (29.7, 60.5) | 7 | 7.0 (0.6, 21.7) | 8 | <0.001 | 0.13 | 0.02 | <0.001 | 0.20 |
Values of variables that did not violate normality are means ± SE, whereas values of variables that violated normality are expressed as medians (25th percentile, 75th percentile); n = no. of rats. Normality of the data was assessed using the Shapiro-Wilk test. PTr, PT, and PTr*T are the outcomes of two-way analysis of variance (ANOVA) with factors treatment (Tr) and time (T) for data that did not violate normality. For data that violated normality, an ANOVA on ranking was performed instead. Dichotomous comparisons of continuous variables were made using Student’s t-test for data that did not violate normality. For data that violated normality, a Mann-Whitney U-test was performed for dichotomous comparisons. P values for dichotomous comparisons were conservatively adjusted using the Dunn-Sidak correction with k = 2 to account for the fact that comparisons were made at 24 h and 5 days. I1, 24 h after ischemia and reperfusion; I5, 5 days after ischemia and reperfusion; S1, 24 h after sham ischemia; S5, 5 days after sham ischemia.