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. 2018 Dec 13;19:27. doi: 10.1186/s12860-018-0178-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Platelet lysis released sufficient amounts of growth factors in PL. a Schematic overview of preparation of PL. PRP was generated from peripheral blood through a series of centrifugations. Based on platelet counts (10⁹ platelets/ml) after the centrifugation step, sufficient PPP was removed to achieve PRP. PL was prepared from PRP by a simple freeze-thawing procedure. b The quantification of growth factors in PL and PPP was determined using ELISA. Bars represented means ± SD. n = 5; *p < 0.05, **p < 0.01, and ***p < 0.005