Table 5.
Glycopeptide MICs of the MITC derivatives that had been introduced mutations in vanB gene cluster
| Strain | MIC (mg/L)a | |
|---|---|---|
| VAN | TEC | |
| M1TC | 3 | 1 |
| M1TC vanSB _E124Ab | 4 | 1 |
| M1TC vanB_ V128Ab | 3 | 1 |
| M1TC vanW_ P92Tb | 3 | 1 |
| M1TC vanSB_ E124A/vanB_ V128Ab | 8 | 1 |
| M1TC vanSB_ E124A/vanW_ P92Tb | 8 | 1 |
| M1TC vanB_ V128A/vanW_ P92Tb | 3 | 1 |
| M1TC vanSB_ E124A/vanB_ V128A/vanW_ P92Tb | 16 | 1 |
aVAN, vancomycin; TEC, teicoplanin bThree unique substitutions for M1 and Y7
255 strains were exchanged to that of the reference gene of pMG2200 by pCJK47-used 256 homologous recombination