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Primer sequences for qRT-PCR
| Gene | Forward primer 5′–3′ | Reverse primer 5′–3′ |
|---|---|---|
| HIF1A-AS2 | AGATCTGTGGCTCAGTTCCTTT | AATCACTATGAATCCCTGCACCT |
| miR-665 | ACCAGGAGGCTGAGGCCCCT | Involved in the kit |
| IL6 | TCAATATTAGAGTCTCAACCCCCA | GAGAAGGCAACTGGACCGAA |
| Runx2 | CCTTCAAGGTGGTAGCCCTC | CCCTAAATCACTGAGGCGGT |
| Osterix (SP7) | AGACCTCCAGAGAGGAGAGAC | GGGGACTGGAGCCATAGTGA |
| Osteocalcin | AATAGCCCTGGCAGATTCCC | CTCTCATGGTGTCTCGGTGG |
| GAPDH | GACAGTCAGCCGCATCTTCT | GCGCCCAATACGACCAAATC |
