Fig. 1.

Inactivation of USF1 enhances cholesterol efflux. a) Cholesterol efflux from THP-1 macrophages treated with non-targeting (NT) and USF1 silencing lentivirus (shUSF1) to wild-type mouse serum, n=6 per condition from two independent experiments. (b) Gene expression of cholesterol transporters relevant for cholesterol efflux in THP-1 macrophages. n=3-4. (c) ABCA1 protein expression in THP-1 cells. n=6/6 from two independent experiments. (d) ABCA1 protein expression in HuH7 hepatoma cells. n=6/6 from two independent experiments. Molecular weights of 220Kda for ABCA1 and 42 kDa for β-actin are depicted in Panels c and d. *** P < 0.001, ** P < 0.01, * P < 0.05, for comparisons between groups