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. Author manuscript; available in PMC: 2019 Feb 14.
Published in final edited form as: Biochem J. 2018 Feb 14;475(3):677–690. doi: 10.1042/BCJ20170672

Figure 6: SRSF1 Mobilization Requires Both SRPK1 and CLK1 Activities.

Figure 6:

A) Confocal microscopy of EGF-treated live HeLa cells expressing GFP-SRSF1 in the absence and presence of TG003. B) Effects of CLK1 knockdown using siRNA on EGF-stimulated mobilization of GFP-SRSF1. C) Treatment of HeLa cells with CLK1 siRNA. Endogenous CLK1 is probed using anti-CLK1 antibody in cell lysates. D) Confocal microscopy of live HeLa cells expressing GFP-SRSF1 in the absence and presence of SRPIN340 and EGF. All speckle areas and total nuclear areas for cells (n=11–18 in (A), 14–20 in (B), and 25–33 in (D)) were calculated using Image J and displayed as a dot plot with mean values ± S.D. P-values were calculated using a one-way ANOVA test. The data in (A) and (D) were generated from replicate experiments. P-values above 0.05 are considered nonsignificant (ns).