Table 1:

Summary of the nuclear actin pools during Drosophila oogenesis.

Labeling tool	Predicted structure	Cell-type	Subnuclear localization	Potential functions
DNase I	G-actin	All cells	Nucleolus	General nucleolar structure and functions. Potential functions include: ribosomal gene expression, ribosome production, and response to cellular stress
Anti-actin C4	G-actin	Anterior escort cells*	Nucleolus	Nucleolar structure and/or function (see above) in cells with or at a times of specific protein production needs
		FSCs
		Some mitotic interphase follicle cells*
		Subset of nurse cells (S3–10)
		Region 3 oocyte
	Polymers	GSCs and early cystoblasts	Nucleoplasm, including chromatin	Maintenance of undifferentiated state via chromatin remodeling and transcriptional programing
		Mitotic follicle cells	Dynamic localization, including to the spindle	Regulation of chromosome segregation
		Oocyte (S3-S13)	Extra chromosomal nucleoplasm	Structural actin meshwork needed for nuclear integrity and organization
Anti-actin AC15	Polymers	Follicle cells (S6-S14)	Chromatin	RNAPII-dependent transcription
		Nurse cells (S6–10)	Chromatin
			Nucleolus	RNAPI- and RNAPIII-dependent transcription
		Migrating border and centripetal follicle cells	Chromatin	MAL/SRF transcription factor regulation
		Oocyte	Puncta near chromosomes	Restricted to puncta and filamentous structures to block oocyte transcription, but have readily available pool of actin for embryonic transcription initiation
			Filamentous structure surrounding chromosomes

^*indicates the cell-types where C4 nuclear actin localizes to the nucleolus, but it also extends slightly beyond it.