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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Neuropeptides. 2018 Oct 26;72:65–74. doi: 10.1016/j.npep.2018.10.003

Figure 3. Effects of MPP or PHTPP Pretreatment on VMN Astrocyte 5’-AMP-Activated Protein Kinase (AMPK) and Phospho-AMPK (pAMPK) Protein Content in Hypoglycemic Female and Male Rats.

Figure 3.

Glial fibrillary acidic protein (GFAP)-immunopositive astrocytes were laser-microdissected from the VMN of vehicle-pretreated eu-(solid white bars) and hypoglycemic (solid gray bars) animals and MPP-(diagonal-striped bars) or PHTPP (cross-hatched gray bars)-pretreated INS-injected rats for Western blot analysis of AMPK [Panel A; female data at left, male data at right] and pAMPK [Panel B; female data at left, male data at right] protein expression. Data illustrate for each sex mean normalized astrocyte protein O.D. measures ± S.E.M. for each treatment group. *p<0.05; **p<0.01; **p<0.001.