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. Author manuscript; available in PMC: 2018 Dec 14.
Published in final edited form as: Oncogene. 2014 Aug 11;34(22):2823–2835. doi: 10.1038/onc.2014.228

Figure 6.

Figure 6.

Tsp-1 is differentially regulated in fibroblasts and epithelial cells. Western blot analysis of (a) immunoblot analysis of Tsp-1, β-Actin and Ras proteins expressed by human dermal fibroblasts expressing hTERT (−), hTERT and SV40 early region (LT) and hTERT, SV40 early region and Ras (Ras). (b) ELISA of secreted VEGF-A by human dermal fibroblasts expressing hTERT (−), hTERT and SV40 early region (LT) and hTERT, SV40 early region and Ras (Ras). (c) Tsp-1 and GAPDH expression in human dermal fibroblasts (hFhT) or lung fibroblasts (MRC5) expressing hTERT alone or with SV40 LTC. (d) Tsp-1 and GAPDH expression in mammary and renal epithelial cells expressing hTERT alone (HME, HRE) or with SV40 LTC (HM-LT, HR-LT). Black lines represent cases where lanes were electronically removed from the scanned image.