Table 1.
Clone | Total length (nt) | BCTIV-derived sequence (nt) [%] | Beet-derived sequence (nt) | BCTIV sequences in minicirclesa | AT % in BCTIV-derived sequence | AT % in beet-derived sequence | Locus of B. vulgaris (best BLAST hit) |
---|---|---|---|---|---|---|---|
RCA from field-grown samples | |||||||
MC#1 | 1315 | 580 [44.1] | 735 | 1–172 … 2435–2845 | 61.4 | 69.8 | Beta chr6 9915362–9916096 |
MC#2 | 1294 | 416 [32.0] | 878b | 1–166 … 2596–2845 | 61.3 | 71.2 | Beta chr7 36583873–36584676 |
MC#3 | 1502 | 444 [29.3] | 1058 | 1–157 … 2223–2299 … 2637–2845 | 59 | 71.2 | Beta chr3:un.sca001 135440–136484 |
RCA from experimental infection | |||||||
MC#4 | 1337 | 459 [34.33] | 878b | 1–135 … 1184–1234 … 2570–2845 | 62.3 | 73.6 | Beta chr6 57105781–57106674 |
MC#5 | 1504 | 532 [35.4] | 972b | 1–267 … 2581–2845 | 62 | 70.6 | Beta chr3.sca001 1755016–1755723 |
MC#6 | 1572 | 574 [36.5] | 998 | 1–188 … 2460–2845 | 61.5 | 68.2 | Beta chr5.sca008 17697504–17698497 |
MC#7 | 1484 | 664 [44.7] | 820 | 1–327 … 2509–2845 | 61.1 | 71.2 | Beta chr1.sca002 10250861–10250043 |
NGS from experimental infection | |||||||
scf #1 | 1407 | 396 [28.0] | 1011b | 1–110 … 2560–2845 | 60.36 | 71.97 | Beta chr4 23770925–23771936 |
scf #2 | 1315 | 332 [25.24] | 983b | 1–177 … 2560–2845 | 60.25 | 73.25 | Beta chr8 1269745–1270665 |
scf #4 | 1336 | 458 [34.28] | 878b | 1–135 … 1184–1234 … 2570–2845 | 62.3 | 73.6 | Beta chr6 57105781–57106674 |
scf #5 | 1552 | 715 [46.1] | 837b | 1–196 … 2327–2845 | 59.31 | 71.45 | Beta chr8 33213612–33212757 |
scf #7 | 1393 | 621 [44.6] | 772b | 1–329 … 2554–2845 | 61.20 | 73.06 | Beta chr6 470978–471718 |
scf#33 | 1241 | 763 [61.48] | 478 | 1–108 … 2188–2845 | 56.49 | 73.44 | Beta 0118.sca00382 22639–23116 |
scf#56/59 | 1429 | 453 [31.7] | 976 | 1–102 … 2495–2845 | 59.83 | 68.04 | Beta chr3 21716285–21716210 |
aBCTIV-Siv (JX082259) coordinates
bIncomplete and/or discontinuous matching