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. 2018 Dec 11;11:8965–8976. doi: 10.2147/OTT.S190303

Figure 6.

Figure 6

ZEB1 expression restored the effects of miR-641 overexpression in the malignant phenotypes of HeLa and SiHa cells.

Notes: The miR-641 mimics-transfected HeLa and SiHa cells were further co-transfected with pc-ZEB1 or pcDNA3.1 blank vector. (A) Western blot analysis was conducted to detect the protein level of HeLa and SiHa cells treated as previously mentioned. The downregulation of ZEB1 protein expression in HeLa and SiHa cells caused by miR-641 overexpression was restored after co-transfection with pc-ZEB1. *P<0.05 vs miR-NC. #P<0.05 vs miR-641 mimics + pcDNA3.1. (B–E) The proliferation, apoptosis, migration, and invasion of previously mentioned cells were examined using CCK-8, flow cytometry, migration, and invasion assays, respectively. ZEB1 reintroduction abolished the inhibitory effects of miR-641 overexpression on HeLa and SiHa cell proliferation, apoptosis, migration, and invasion. *P<0.05 vs miR-NC. #P<0.05 vs miR-641 mimics + pcDNA3.1.

Abbreviations: CCK-8, Cell counting kit-8; miR-641, miRNA-641; miR-NC, negative control miRNA mimics.