Figure 5.

Overexpression of ILF3 promotes melanoma proliferation, migration, and invasion.

Notes: (A) Twenty-four hours after transiently transfecting ILF3 overexpression plasmids into SK-MEL-2 cells, cell viabilities of SK-MEL-2 cells were determined by the Glo cell viability assay. (B) Forty-eight hours after transiently transfecting ILF3 overexpression plasmids into SK-MEL-2 cells, cell proliferation of SK-MEL-2 cells was determined by the EdU incorporation assay. The red color indicates EdU-positive nuclei. Scale bars, 100 µm. (C) Forty-eight hours after transiently transfecting ILF3 overexpression plasmids into SK-MEL-2 cells, cell migration of SK-MEL-2 cells was determined by transwell migration assay. Scale bars, 100 µm. (D) Forty-eight hours after transiently transfecting ILF3 overexpression plasmids into SK-MEL-2 cells, cell invasion of SK-MEL-2 cells was determined by transwell invasion assay. Scale bars, 100 µm. Results are presented as mean ± SD based on at least three independent experiments. ^**P<0.01 by Student’s t-test.

Abbreviation: EdU, ethynyl deoxyuridine.