Figure 6.

Knockdown of ILF3 inhibits melanoma proliferation, migration, and invasion.

Notes: (A) Twenty-four hours after transiently transfecting two independent ILF3-specific shRNAs into A375 cells, cell viabilities of A375 cells were determined by the Glo cell viability assay. (B) Forty-eight hours after transiently transfecting two independent ILF3-specific shRNAs into A375 cells, cell proliferation of A375 cells was determined by the EdU incorporation assay. The red color indicates EdU-positive nuclei. Scale bars, 100 µm. (C) Forty-eight hours after transiently transfecting two independent ILF3-specific shRNAs into A375 cells, cell migration of A375 cells was determined by transwell migration assay. Scale bars, 100 µm. (D) Forty-eight hours after transiently transfecting two independent ILF3-specific shRNAs into A375 cells, cell invasion of A375 cells was determined by the transwell invasion assay. Scale bars, 100 µm. Results are presented as mean ± SD based on at least three independent experiments. ^**P<0.01; ^***P<0.001 by one-way ANOVA.

Abbreviation: EdU, ethynyl deoxyuridine.