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. 2018 Dec 10;13:8395–8410. doi: 10.2147/IJN.S188016

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© 2018 Shobaki et al. This work is published and licensed by Dove Medical Press Limited

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In vivo silencing activity of LNPs in hepatocytes and LSECs.

Notes: Mice were intravenously injected with LNPs encapsulating a mixture of siRNA (siCD31:siFVII 1:1 ratio) at a dose of 0.1 mg/kg of each type of siRNA. After 24 hours, levels of the FVII protein in plasma (A) and CD31 mRNA in liver tissues (B) were measured by means of an FVII assay and qRT-PCR, respectively. The reference gene in LSECs was TIE2. ^**P<0.01, nonrepeated ANOVA followed by an SNK test; data represent the mean±SD (n=3).

Abbreviations: FVII, coagulation factor VII; LNP, lipid nanoparticle; LSECs, liver sinusoidal endothelial cells; mRNA, messenger RNA; pKa, acid dissociation constant; qRT-PCR, quantitative real-time polymerase chain reaction; siRNA, short interfering RNA; TIE2, angiopoietin receptor.