Table S3.
Physical characteristics of the LNPs
| Lipid composition
|
pKa | Size (nm) | Number mean (nm) | PDI | ζ-potential (mV) | EE of siRNA (%) | |
|---|---|---|---|---|---|---|---|
| YSK05 (mol%) | YSK12-C4 (mol%) | ||||||
|
| |||||||
| 70 | 0 | 6.50 | 84.02 ± 6.82 | 58.52 ± 3.49 | 0.13 ± 0.03 | 1.70 ± 4.59 | 94.82 ± 0.64 |
| 50 | 20 | 7.15 | 85.59 ± 8.22 | 55.71 ± 2.91 | 0.17 ± 0.08 | 6.12 ± 1.46 | 95.32 ± 1.12 |
| 35 | 35 | 7.50 | 84.89 ± 11.79 | 56.31 ± 7.80 | 0.14 ± 0.08 | 7.75 ± 2.83 | 96.18 ± 1.73 |
| 20 | 50 | 7.75 | 92.86 ± 10.27 | 56.55 ± 8.01 | 0.19 ± 0.04 | 8.97 ± 1.74 | 97.58 ± 0.96 |
| 0 | 70 | 8.00 | 92.61 ± 11.47 | 51.72 ± 0.99 | 0.22 ± 0.10 | 11.63 ± 1.56 | 97.41 ± 0.67 |
Notes: After preparing LNPs that have the composition YSK05/YSK12 C4/Cholesterol/mPEG2k-DMG=(70-X):X:30:2 mol% of total lipids (X=0~70) by t-BuOH dilution method, the pKa was measured using a TNS assay; size, number mean, PDI, and ζ-potential were measured using DLS; EE of siRNA was measured using RiboGreen assay. Data represent mean±SD (n=3).
Abbreviations: DLS, dynamic light scattering; EE, encapsulation efficiency; LNP, lipid nanoparticle; PDI, polydispersity index; mPEG2k-DMG, 1,2-Dimirystoyl-sn-3-glycero methoxypolyethyleneglycol 2000 ether; pKa, acid dissociation constant; siRNA, short interfering RNA; t-BuOH, tertiary butanol; TNS, 6-(p-toluidino)-2-naphthalenesulfonic acid.