Figure 2.

Satellite Cell-specific Rpt3-Knockout Mice Exhibit No Obvious Skeletal Muscle Phenotype

(A) Time course for tamoxifen (Tmx) treatment and tissue harvesting. Con indicates Rpt3^f/f mice and scKO indicates satellite cell-specific Rpt3-knockout mice (Pax7^CreERT2/+; Rpt3^f/f).

(B) Relative expression of Rpt3 mRNA in freshly isolated satellite cells derived from Con and scKO mice after Tmx injection. Data represent means ± SEM (t test: ^∗∗∗p < 0.001; n = 4 per group). AU, arbitrary units.

(C) Chymotrypsin-like and trypsin-like proteasome activities (relative to Con) in freshly isolated satellite cells derived from Con and scKO mice after Tmx injection. Data represent means ± SEM (t test: ^∗∗p < 0.01; n = 4–5 per group). IU, international units.

(D) Change in body weight (g) after Tmx injection. Data represent mean ± SD (NS, statistically nonsignificant, n = 5–10 per group).

(E) Change in tibialis anterior (TA) muscle weight (g) at 2 months after Tmx injection. Data represent mean ± SD (NS, statistically nonsignificant, n = 4–6 per group).

(F) H&E staining of intact TA muscle 2 months after Tmx injection. Scale bar, 50 μm. Also shown in Figure S1D.

(G) Endurance time(s) of Con and scKO mice. Data represent mean ± SD (NS, statistically nonsignificant, n = 4–6 per group).

See also Figures S2–S4.