Figure 5.
Deficiency of Rpt3 in Satellite Cells Results in Proliferative Defects during Muscle Regeneration In Vivo
(A) Time course for tamoxifen (Tmx) treatment and tissue harvesting. Con indicates Rpt3f/f mice and scKO indicates satellite cell-specific Rpt3 knockout mice (Pax7CreERT2/+; Rpt3f/f).
(B) Immunostaining for Cleaved-Caspase3 (green), Pax7 (red), and DAPI (blue) in tibialis anterior (TA) muscle cryosections 3 days after Tmx treatment. Arrows indicate satellite cells. Scale bar, 10 μm. The y axis shows the ratio of Cleaved-Caspase3-positive cells to PAX7-positive cells. Data represent means ± SD (t test: ∗∗p < 0.01; n = 3 per group).
(C) Time course for Tmx treatment and tissue harvesting.
(D) Immunostaining for EdU (green), M-cadherin (red), and DAPI (blue) in TA muscle cryosections, 3 days after Tmx treatment. Arrows indicate satellite cells. Scale bar, 10 μm. The y axis shows the ratio of EdU-positive cells to M-cadherin-positive cells. Data represent means ± SD (t test: NS, no significance; n = 3 per group).
(E) Immunostaining for M-cadherin (green), KI67 (red), and DAPI (blue) in TA muscle cryosections, 3 days after Tmx treatment. Arrows indicate satellite cells. Scale bar, 10 μm. The y axis shows the ratio of KI67-positive cells to M-cadherin-positive cells. Data represent means ± SD (t test: NS, no significance; n = 3 per group).
(F) Time course for Tmx treatment and tissue harvesting.
(G) Immunostaining for EdU (green), M-cadherin (red), and DAPI (blue) in TA muscle cryosections, 3 days after injury. Scale bar, 50 μm. The y axis shows the number of EdU-positive and M-cadherin-positive cells per field. Data represent means ± SD (t test: ∗∗p < 0.01; n = 3 per group).