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. 2018 Nov 29;11(6):1493–1505. doi: 10.1016/j.stemcr.2018.11.002

Figure 2.

Figure 2

Histopathological Characterization of Xenografts Derived from Different hPSC and hGCT Cell Lines

(A–G) Representative pictures of H&E and immunohistochemistry of xenografts derived from hPSCs (A) H9, (B) H9 + Dox, (C) H9Hyb, (D) Lu07, (E) Lu07 + Dox and hGCT-derived cell lines, (F) 2102EpL, and (G) TCam-2. H&E-stained xenografts (A–D) show differentiated tissue areas representing derivatives of mesoderm (Meso), ectoderm (Ecto), and endoderm (Endo). Scale bars, 200 μm. Representative images of immunohistochemistry for the pluripotency markers OCT4 and specific markers for hGCTs SOX17, SOX2, CD30, and GPC3 are shown. Nuclei were counterstained with hematoxylin only. Scale bars, 100 μm (for the immunohistochemistry images) (see Table S3 for antibody list).

(H) Summary of presence of tissues derived from the three embryonic germ layers, undifferentiated embryonal carcinoma (EC) cells, yolk sac (YS) elements, and seminoma (SE) components in the xenograft. Notice that Lu07, although negative for all other markers, showed small groups of cells positive for GPC3 (i.e., YS differentiation).